Optimization of Liposomal Formulations Using Design of Experiments (DOE) For Enhanced Skin Penetration of Anti-Aging Compounds

Liposomal drug delivery systems have been developed as a potential favourite to ameliorate the dermal delivery of the anti-aging substances because they are biocompatible, have the capacity to bind both hydrophilic and lipophilic drugs and possess the ability to boost targeted treatments. In this work the systematic study was done to optimize liposomal formulations against skin penetration by application of Design of Experiments (DoE) approach. The factorial design was used to optimize various formulation parameters such as phospholipid concentration, cholesterol content and hydration time and their influence on the effect on vesicle size, entrapment efficiency and in-vitro skin penetration established. The statistical analysis indicated there was important interaction among the variables and therefore optimal formulation was defined using the technique and it performed better in terms of skin permeation behavior. The findings indicate the effectiveness of the DoE strategy in the development of formulation and the potential of optimized liposomes as a delivery vehicle to anti-aging products.